抗菌肽Sublancin与黄芪多糖对免疫抑制小鼠免疫功能调节作用的比较分析

doi:10.11843/j.issn.0366-6964.2019.02.019

Abstract

Abstract:

The purpose of this study was to evaluate the immunomodulatory effects of antimicrobial peptide sublancin and astragalus polysaccharides (APS) on immunosuppressive mice induced by cyclophosphamide (CTX). Sixty healthy female BALB/c mice aged 4-6 weeks were randomly divided into 6 groups of 10 each. Normal control group:d 1 to d 3, intraperitoneally injected with 0.9% physiological saline, d 4 to d 10, gavage 0.9% physiological saline. The other 5 groups:d 1 to d 3, injected with CTX (80 mg·kg^-1). On the d 4 to d 10, the negative control group:gavage 0.9% physiological saline; low or high concentration sublancin treatment group:sublancin solution was administered at a dose of 4.0 and 8.0 mg·kg^-1; the APS-treated group:gavage APS (200 mg·kg^-1); positive control group:gavage levamisole (10 mg·kg^-1, BW). All treatments were performed once a day and 0.2 mL each time. The mice were weighed before the mice were intraperitoneally injected with CTX on the first day of the experiment, on the d 4 before gavage and on the d 11. After weighing the body weight on the d 11, peripheral blood and spleen were collected. Compared with the normal control group, the peripheral blood red blood cell, hemoglobin, and white blood cell counts in the negative control group were significantly reduced (P<0.05); the gene expression of IL-2, IL-4, and IL-6 in the spleen was significantly reduced (P<0.05). Compared with the negative control group, there was no difference in body weight between the APS group and the high concentration Sublancin group after intragastric administration but there is an upward trend, indicating that high concentration Sublancin and APS had a positive effect on body weight of mice; the leukocyte content in peripheral blood of low and high concentration Sublancin, APS, and positive control groups were significantly increased (P<0.05); in the high concentration Sublancin-treated group, red blood cells and hemoglobin were significantly increased (P<0.05); CD4⁺ levels in peripheral blood of mice treated with low or high concentration Sublancin and APS were significantly increased (P<0.05); in both low and high concentration Sublancin, APS and positive control groups, CD8⁺ was significantly decreased (P<0.05); in low and high concentration sublancin groups, CD4⁺/CD8⁺ was significantly increased (P<0.05); in low and high concentration sublancin, APS and positive control groups, mouse spleen IL-4 were significantly increased (P<0.05); in high concentration sublancin, APS and positive control groups, the expressions of IL-2 and IL-6 in the spleen of mice were significantly increased (P<0.05). In summary, it can be concluded that appropriate doses of the antimicrobial peptides sublancin and APS can all alleviate the immunosuppression caused by cyclophosphamide. Compared with APS, the role of antimicrobial peptide sublancin in the effect on alleviating the immunosuppression of CTX is more comprehensive.

CLC Number:

S852.4

SHANG Lijun, YANG Tianren, YU Haitao, HUANG Shuo, ZENG Xiangfang, QIAO Shiyan. Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 406-414.

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Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study

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