抗菌肽Sublancin与黄芪多糖对免疫抑制小鼠免疫功能调节作用的比较分析

doi:10.11843/j.issn.0366-6964.2019.02.019

Abstract

Abstract:

The purpose of this study was to evaluate the immunomodulatory effects of antimicrobial peptide sublancin and astragalus polysaccharides (APS) on immunosuppressive mice induced by cyclophosphamide (CTX). Sixty healthy female BALB/c mice aged 4-6 weeks were randomly divided into 6 groups of 10 each. Normal control group:d 1 to d 3, intraperitoneally injected with 0.9% physiological saline, d 4 to d 10, gavage 0.9% physiological saline. The other 5 groups:d 1 to d 3, injected with CTX (80 mg·kg^-1). On the d 4 to d 10, the negative control group:gavage 0.9% physiological saline; low or high concentration sublancin treatment group:sublancin solution was administered at a dose of 4.0 and 8.0 mg·kg^-1; the APS-treated group:gavage APS (200 mg·kg^-1); positive control group:gavage levamisole (10 mg·kg^-1, BW). All treatments were performed once a day and 0.2 mL each time. The mice were weighed before the mice were intraperitoneally injected with CTX on the first day of the experiment, on the d 4 before gavage and on the d 11. After weighing the body weight on the d 11, peripheral blood and spleen were collected. Compared with the normal control group, the peripheral blood red blood cell, hemoglobin, and white blood cell counts in the negative control group were significantly reduced (P<0.05); the gene expression of IL-2, IL-4, and IL-6 in the spleen was significantly reduced (P<0.05). Compared with the negative control group, there was no difference in body weight between the APS group and the high concentration Sublancin group after intragastric administration but there is an upward trend, indicating that high concentration Sublancin and APS had a positive effect on body weight of mice; the leukocyte content in peripheral blood of low and high concentration Sublancin, APS, and positive control groups were significantly increased (P<0.05); in the high concentration Sublancin-treated group, red blood cells and hemoglobin were significantly increased (P<0.05); CD4⁺ levels in peripheral blood of mice treated with low or high concentration Sublancin and APS were significantly increased (P<0.05); in both low and high concentration Sublancin, APS and positive control groups, CD8⁺ was significantly decreased (P<0.05); in low and high concentration sublancin groups, CD4⁺/CD8⁺ was significantly increased (P<0.05); in low and high concentration sublancin, APS and positive control groups, mouse spleen IL-4 were significantly increased (P<0.05); in high concentration sublancin, APS and positive control groups, the expressions of IL-2 and IL-6 in the spleen of mice were significantly increased (P<0.05). In summary, it can be concluded that appropriate doses of the antimicrobial peptides sublancin and APS can all alleviate the immunosuppression caused by cyclophosphamide. Compared with APS, the role of antimicrobial peptide sublancin in the effect on alleviating the immunosuppression of CTX is more comprehensive.

CLC Number:

S852.4

SHANG Lijun, YANG Tianren, YU Haitao, HUANG Shuo, ZENG Xiangfang, QIAO Shiyan. Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 406-414.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2019.02.019

https://www.xmsyxb.com/EN/Y2019/V50/I2/406

References

[1] ZASLOFF M. Antimicrobial peptides of multicellular organisms[J]. Nature, 2002, 415(6870):389-395.
[2] LAI Y P, GALLO R L. AMPed up immunity:how antimicrobial peptides have multiple roles in immune defense[J]. Trends Immunol, 2009, 30(3):131-141.
[3] MANSOUR S C, PENA O M, HANCOCK R E W. Host defense peptides:front-line immunomodulators[J]. Trends Immunol, 2014, 35(9):443-450.
[4] PAIK S H, CHAKICHERLA A, HANSEN J N. Identification and characterization of the structural and transporter genes for, and the chemical and biological properties of, sublancin 168, a novel lantibiotic produced by Bacillus subtilis 168[J]. J Biol Chem, 1998, 273(36):23134-23142.
[5] KOUWEN T R H M, TRIP E N, DENHAM E L, et al. The large mechanosensitive channel MscL determines bacterial susceptibility to the bacteriocin sublancin 168[J]. Antimicrob Agents Chemother, 2009, 53(11):4702-4711.
[6] 王帅. 抗菌肽Sublancin对肉鸡坏死性肠炎和小鼠天然免疫的影响[D]. 北京:中国农业大学, 2017.
WANG S. Effects of antimicrobial peptide Sublancin on necrotic enteritis of broilers and the innate immunity of mice[D]. Beijing:China Agricultural University, 2017. (in Chinese)
[7] 张小梅. 黄芪多糖的免疫调节作用及抗肿瘤作用研究进展[J]. 大连大学学报, 2003, 24(6):101-104.
ZHANG X M. Advances in the immunomodulatory effects and anti-tumor effects of Astragalus polysaccharides[J]. Journal of Dalian University, 2003, 24(6):101-104. (in Chinese)
[8] LIU Q Y, YAO Y M, YU Y, et al. Astragalus polysaccharides attenuate postburn sepsis via inhibiting negative immunoregulation of CD4⁺ CD25^high T cells[J]. PLoS One, 2011, 6(7):e19811.
[9] JIANG R Z, WANG Y, LUO H M, et al. Effect of the molecular mass of tremella polysaccharides on accelerated recovery from cyclophosphamide-induced leucopenia in rats[J]. Molecules, 2012, 17(12):3609-3617.
[10] HATTA H, TSUDA K, AKACHI S, et al. Productivity and some properties of egg yolk antibody (IgY) against human rotavirus compared with rabbit IgG[J]. Biosci Biotechnol Biochem, 1993, 57(3):450-454.
[11] PICHEL N, VIVARB M. Origin of thyroid dysfunction at the Saharawi refugee camps[J]. J Trace Elem Med Biol, 2018, 48:57.
[12] LIAO W, LIN J X, LEONARD W J. IL-2 family cytokines:new insights into the complex roles of IL-2 as a broad regulator of T helper cell differentiation[J]. Curr Opin Immunol, 2011, 23(5):598-604.
[13] PARK H Y, YU A R, CHOI I W, et al. Immunostimulatory effects and characterization of a glycoprotein fraction from rice bran[J]. Int Immunopharmacol, 2013, 17(2):191-197.
[14] TZIANABOS A O. Polysaccharide immunomodulators as therapeutic agents:structural aspects and biologic function[J]. Clin Microbiol Rev, 2000, 13(4):523-533.
[15] 李波,韩文瑜,宋智娟,等. 抗菌肽的免疫调节活性及其在动物生产中的应用[J]. 中国饲料, 2014(4):14-17.
LI B, HAN W Y, SONG Z J, et al. Immunoregulatory of antimicrobial peptide and its application in animal production[J]. China Feed, 2014(4):14-17. (in Chinese)
[16] WANG S, WANG Q W, ZENG X F, et al. Use of the antimicrobial peptide Sublancin with combined antibacterial and immunomodulatory activities to protect against methicillin-resistant Staphylococcus aureus infection in mice[J]. J Agric Food Chem, 2017, 65(39):8595-8605.
[17] LIU Y, ZHANG S F, ZHANG F, et al. Adjuvant activity of Chinese herbal polysaccharides in inactivated veterinary rabies vaccines[J]. Int J Biol Macromol, 2012, 50(3):598-602.
[18] 赵弋清,罗霞,陈东辉,等. 不同剂量环磷酰胺诱导正常小鼠免疫抑制的对比研究[J]. 免疫学杂志, 2005, 21(3):122-124, 128.
ZHAO Y Q, LUO X, CHEN D H, et al. Comparison of immunosuppression induced by different doses of cyclophosphamide in normal mice[J]. Immunological Journal, 2005, 21(3):122-124, 128. (in Chinese)
[19] EL-ABASY M, MOTOBU M, NAKAMURA K, et al. Preventive and therapeutic effects of sugar cane extract on cyclophosphamide-induced immunosuppression in chickens[J]. Int Immunopharmacol, 2004, 4(8):983-990.
[20] CRUZ-CHAMORRO L, PUERTOLLANO M A, PUERTOLLANO E, et al. Examination of host immune resistance against Listeria monocytogenes infection in cyclophosphamide-treated mice after dietary lipid administration[J]. Clin Nutrit, 2007, 26(5):631-639.
[21] ATSAMO A D, NGUELEFACK T B, DATTÉ J Y, et al. Acute and subchronic oral toxicity assessment of the aqueous extract from the stem bark of Erythrina senegalensis DC (Fabaceae) in rodents[J]. J Ethnopharmacol, 2011, 134(3):697-702.
[22] SHEEJA K, KUTTAN G. Ameliorating effects of Andrographis paniculata extract against cyclophosphamide-induced toxicity in mice[J]. Asian Pac J Cancer Prev, 2006, 7(4):609-614.
[23] SHALIT I, KLETTER Y, HALPERIN D, et al. Immunomodulatory effects of moxifloxacin in comparison to ciprofloxacin and G-CSF in a murine model of cyclophosphamide-induced leukopenia[J]. Eur J Haematol, 2001, 66(5):287-296.
[24] BERGER M, LANINO E, CESARO S, et al. Feasibility and outcome of haploidentical hematopoietic stem cell transplantation with post-transplant high-dose cyclophosphamide for children and adolescents with hematologic malignancies:an AIEOP-GITMO retrospective multicenter study[J]. Biol Blood Marrow Transplant, 2016, 22(5):902-909.
[25] ZHAO L, ASHRAF M A. Influence of silver-hydroxyapatite nanocomposite coating on biofilm formation of joint prosthesis and its mechanism[J]. West Indian Med J, 2015, 64(5):506-513.
[26] TINDEMANS I, SERAFINI N, DI SANTO J P, et al. GATA-3 function in innate and adaptive immunity[J]. Immunity, 2014, 41(2):191-206.
[27] LIU Q, YU S, LI A, et al. Targeting interlukin-6 to relieve immunosuppression in tumor microenvironment[J]. Tumour Biol, 2017, 39(6):1010428317712445.
[28] NAKA T, NISHIMOTO N, KISHIMOTO T. The paradigm of IL-6:from basic science to medicine[J]. Arthritis Res, 2002, 4(S3):S233-S242.
[29] TANIGUCHI K, KARIN M. IL-6 and related cytokines as the critical lynchpins between inflammation and cancer[J]. Semin Immunol, 2014, 26(1):54-74.
[30] GUTHRIE G J K, ROXBURGH C S D, RICHARDS C H, et al. Circulating IL-6 concentrations link tumour necrosis and systemic and local inflammatory responses in patients undergoing resection for colorectal cancer[J]. Br J Cancer, 2013, 109(1):131-137.
[31] HOU H, FAN Y, WANG S K, et al. Immunomodulatory activity of Alaska pollock hydrolysates obtained by glutamic acid biosensor-Artificial neural network and the identification of its active central fragment[J]. J Funct Foods, 2016, 24:37-47.
[32] 张琰,程建峰,贺建荣,等. 黄芪多糖对环磷酰胺致小鼠骨髓抑制及毒性的保护作用[J]. 第四军医大学学报, 2003, 24(5):447-448.
ZHANG Y, CHENG J F, HE J R, et al. Protection of astragalus polyaaccharidcs on toxicity and bone marrow inhibition caused by cyclophosphamide[J]. Journal of the Fourth Military Medical University, 2003, 24(5):447-448. (in Chinese)
[33] ZHU Y L, WANG L Y, WANG J X, et al. Protective effects of paeoniflorin and albiflorin on chemotherapy-induced myelosuppression in mice[J]. Chin J Nat Med, 2016, 14(8):599-606.
[34] 徐尚福,杨天华,吴芹,等. 黄芪多糖对环磷酰胺引起小鼠骨髓抑制后造血功能的修复作用[J]. 遵义医学院学报, 2011, 34(5):473-476.
XU S F, YANG T H, WU Q, et al. Restorative effect of astragalus polysaccharides on hematopoietic function in cyclophosphamide-induced bone marrow depression in mice[J]. Acta Academiae Medicinae Zunyi, 2011, 34(5):473-476. (in Chinese)
[35] 李秀,初杰,李影迪,等. 当归/黄芪多糖对腹腔注射环磷酰胺小鼠骨髓造血影响随机平行对照研究[J]. 实用中医内科杂志, 2017, 31(5):52-58.
LI X, CHU J, LI Y D, et al. Angelica sinensis/astragalus polysaccharides on hematopoietic function of bone barrow of mice induced by intraperitoneal injection of cyclophosphamide impact a randomized parallel controlled study[J]. Journal of Practical Traditional Chinese Internal Medicine, 2017, 31(5):52-58. (in Chinese)
[36] SYRJÄLÄ H, SURCEL H M, ILONEN J. Low CD4/CD8 T lymphocyte ratio in acute myocardial infarction[J]. Clin Exp Immunol, 1991, 83(2):326-328.
[37] MCBRIDE J A, STRIKER R. Imbalance in the game of T cells:what can the CD4/CD8 T-cell ratio tell us about HIV and health?[J]. PLoS Pathog, 2017, 13(11):e1006624.
[38] TAKEUCHI A, ETO M, YAMADA H, et al. A reduction of recipient regulatory T cells by cyclophosphamide contributes to an anti-tumor effect of nonmyeloablative allogeneic stem cell transplantation in mice[J]. Int J Cancer, 2012, 130(2):365-376.
[39] LIU P, JAFFAR J, HELLSTROM I, et al. Administration of cyclophosphamide changes the immune profile of tumor-bearing mice[J]. J Immunother, 2010, 33(1):53-59.
[40] GHIRINGHELLI F, LARMONIER N, SCHMITT E, et al. CD4⁺ CD25⁺ regulatory T cells suppress tumor immunity but are sensitive to cyclophosphamide which allows immunotherapy of established tumors to be curative[J]. Eur J Immunol, 2004, 34(2):336-344.
[41] HONG S H, YOON I H, KIM Y H, et al. High-dose cyclophosphamide-mediated anti-tumor effects by the superior expansion of CD44^high cells after their selective depletion[J]. Immunobiology, 2010, 215(3):182-193.
[42] BRODE S, RAINE T, ZACCONE P, et al. Cyclophosphamide-induced type-1 diabetes in the NOD mouse is associated with a reduction of CD4⁺ CD25⁺ Foxp3⁺ regulatory T cells[J]. J Immunol, 2006, 177(10):6603-6612.
[43] LUTSIAK M E, SEMNANI R T, DE RASCALIS P, et al. Inhibition of CD4⁺ 25⁺ T regulatory cell function implicated in enhanced immune response by low-dose cyclophosphamide[J]. Blood, 2005, 105(7):2862-2868.

Immunomodulation Effect of Antimicrobial Peptide Sublancin and Astragalus Polysaccharides on Immunosuppressed Mice: A Comparative Study

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	BAI Yun, XIE Qingyun, OUYANG Wei, GAN Yuan, YUAN Ting, ZHAO Dongming, BU Zhigao, SHAO Guoqing, FENG Zhixin. Establishment of a Serological Method for Early Detection of African Swine Fever Virus Infection Based on Mucosal sIgA Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 300-310.
[2]	GONG Haoyang, WU Jiaxin, YANG Xiaoyu, XIE Weichun, WANG Xueying, LI Jiaxuan, JIANG Yanping, CUI Wen, LI Yijing, TANG Lijie. Research Progress in Antiviral Mechanism of Intestinal Microflora [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4910-4919.
[3]	ZHENG Ruoyu, XIAO Jie, BAI Xin, CHEN Hao, PU Jiayan, REN Yongjun, YANG Guangyou. Evaluation of the Immunoprotective Effect of Recombinant Protein of Eimeria magna 3-Phosphoglyceraldehyde Dehydrogenase on Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4338-4349.
[4]	WANG Jiandong, TANG Yulin, WANG Min, ZHANG Baosuo, YANG Fuqiang, GAO Haihui, YU Yang, GUO Yansheng. The Mechanism of Lycium barbarum Polysaccharide against Immunosuppression Induced by Cyclophosphamide in Chicks Based on RNA-Seq Technique [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3519-3532.
[5]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[6]	LIU Hongyi, LUO Tingyu, LI Changwen, YU Haibo, LU Xiaoye, CHEN Hongyan, XIA Changyou, GAO Caixia. Identification of SLA-1 Alleles and Analysis of Molecular Genetic Characteristics in Rongchang Pigs [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3064-3077.
[7]	LI Zhaoyan, GAO Jiang, GUO Shihui, ZHAO Ruqian, MA Wenqiang. Advances in Detection Methods and Control Solutions for Feline Allergens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2272-2279.
[8]	ZHENG Ruoyu, REN Yongjun, XIAO Jie, BAI Xin, PU Jiayan, CHEN Hao, YANG Guangyou. Evaluation of the Immunoprotective Effect of Recombinant Protein of Eimeria stiedae 3-Phosphoglyceraldehyde Dehydrogenase on Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2581-2595.
[9]	LIU Qing, WU Shaopeng, SHI Bin, SHAO Hongxia, QIAN Kun, YE Jianqiang, QIN Aijian. Expression of Chicken CTLA-4 Protein in Insect Cells and the Preparation of Its Monoclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2596-2604.
[10]	CHEN Fangfang, LI Zhonghua, ZHU Zhiwei, LI Jinchun, LIU Cuiyan. Recent Advances in Multifunctional Research of Invariant Chain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1824-1833.
[11]	WANG Zi, WANG Nianxiang, TIAN Changming, ZHAO Fujie, LIU Lintao, MA Mengyao, JIA Xinhao, LIU Guoxing, ZHENG Lanlan. Using Mouse to Evaluate the Immune Effect of Bridged Diphenylalanine Dipeptide with Inactivated Porcine Deltacoronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1590-1597.
[12]	LI Shuo, ZHANG Yun, BAI Manyuan, ZHAO Ruichong, SONG Hetao, MU Suyu, TENG Zhidong, DONG Hu, MA E'ning, SUN Shiqi, GUO Huichen, YIN Shuanghui. Immunogenicity Evaluation of Biomineralized Foot-and-mouth Disease Virus-like Particles Vaccine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1598-1607.
[13]	LI Rang, WENG Xiang, LI Quanxiao, WU Daocheng, CAO Hui, ZHANG Ailian. Analysis of Emulsifying Method and Stability of Foot-and-mouth Disease Vaccine Combined with Crude Polysaccharides from Cultivated Artemisia rupestris L. [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1608-1615.
[14]	HE Haojie, XUE Mei, FENG Li. Activation Mechanism of NLRP1 Inflammasome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 494-503.
[15]	PAN Weixiong, CHEN Yuyi, ZHAO Zengjue, FENG Saixiang, QIU Weihong, YE Hejia, ZHANG Linghua. Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4367-4378.